RNA size markers

Up-Regulation of TPT1-AS1 and SAMMSON and Down-Regulation of LINC00961 Long Non-Coding RNAs (lncRNAs) as Potential Tumor Markers in Gastric Cancer

Background and Objective:Gastric Cancer (GC) is one of the deadliest cancers in the world. Recently, LINC00961, TPT1-AS1, and SAMMSON Long non-coding RNA (lncRNAs)have been discovered, which significantly contribute to the occurrence of various cancers. This study aimed to determine the expression levels of these genes in GC tissues, compared to healthy adjacent tissues, and the relationship of...

Generation of RNA ladders by rolling circle transcription of small circular oligodeoxyribonucleotides.

As experimental methods in molecular biology have rapidly progressed, the need for simple methodology to create RNA size markers has become increasingly apparent. Ribosomal RNAs are commonly used as size markers for larger RNAs in denaturing agarose gels (9); however, RNA markers are not commercially available for the characterization of <100 nucleotide (nt) RNAs by electrophoresis. This fact, ...

PreRkTAG: Prediction of RNA Knotted Structures Using Tree Adjoining Grammars

Background: RNA molecules play many important regulatory, catalytic and structural <span style="font-variant: normal; font-style: norma...

A new method for the size estimation of the RNA genome segments of influenza virus.

Previous estimates of the size of the RNA genome segments of influenza virus have been unreliable because of a lack of suitable RNA species as size markers. We have attempted to overcome this problem by utilising the ability of AMV reverse transcriptase to synthesise full length DNA copies of RNA molecules in the presence of a suitable primer. By comparing such DNA copies of the RNA segments of...

Design and use of easily made RNA size markers.

Genetic Heterogeneity among Leishmania major Isolates in Iran Determined by Restriction Fragment Length Polymorphism (RFLP) and Multilocus Microsatellite Typing (MLMT)

Background & Aims: In recent years, molecular methods for characterizing genetic heterogeneity have found a major place in modern approaches. In this study, two different molecular techniques including Restriction Fragment Length Polymorphism (RFLP) and Multi Locus microsatellite typing (MLMT) were carried out in order to evaluate genetic heterogeneity among isolates of Leishmania major in Iran...